Evaluating Dye-Labeled DNA Dendrimers for Potential Applications in Molecular Biosensing

• DOI: 10.1021/acssensors.6b00778
• Publication Date: 2017-03-09T15:32:17Z
• Authors: Carl W. Brown; Susan Buckhout-White; Sebastián A. Díaz; Joseph S. Melinger; Mario G. Ancona; Ellen R. Goldman; Igor L. Medintz
• Keywords: Transduction (biophysics)

DNA nanostructures provide a reliable and predictable scaffold for precisely positioning fluorescent dyes to form energy transfer cascades. Furthermore, these structures their attendant dye networks can be dynamically manipulated by biochemical inputs, with the changes reflected in spectral response. However, complexity of that have undergone such types manipulation direct biosensing applications is quite limited. Here, we investigate four different modification strategies effect dynamic manipulations using dendrimer as testbed, mind. The has 2:1 branching ratio organizes into FRET-based antenna which excitonic generated on multiple initial Cy3 displayed at periphery then transferred inward through Cy3.5 and/or Cy5 relay Cy5.5 final acceptor focus. Advantages this design included good efficiency, large separation between donor emissions signal transduction, an inherent tolerance defects. Of approaches structural rearrangement, first two mechanisms consider employed either toehold-mediated strand displacement or replacement impact was mainly via while other were more global belting mechanism 8-arm star nanostructure compress thereby modulate its response enhancement parallelism. performance mechanisms, ability reset, how they might utilized are discussed.