Dark-Field Microwells toward High-Throughput Direct miRNA Sensing with Gold Nanoparticles

• DOI: 10.1021/acssensors.9b00946
• Publication Date: 2019-07-16T14:06:58Z
• Authors: Stephanie Hwu; Yves Blickenstorfer; Raphael F. Tiefen...; Claudio Gonnelli; Lukas Schmidheini; Ines Lüchtefeld; Bas-Jan Hoogenberg; Andrea B. Gisiger; János Vörös
• Keywords: Point of care

MicroRNA (miRNA) is a class of short RNA that emerging as an ideal biomarker, its expression level has been found to correlate with different types diseases including diabetes and cancer. The detection miRNA highly beneficial for early diagnostics disease monitoring. However, sensing remains difficult because small size low levels. Common techniques such quantitative real-time polymerase chain reaction (qRT-PCR), in situ hybridization Northern blotting have developed quantify given sample. Nevertheless, these methods face common challenges point-of-care practice they either require complicated sample handling expensive equipment, or suffer from sensitivity. Here we present new tool based on dark-field microwells overcome sensing. This miniaturized device enables the readout gold nanoparticle assay without need microscope. We demonstrate feasibility detect both buffer solution cell lysate. allow affordable at high throughput which make them promising diagnostics.