Smartphone-Based Point-of-Care Microfluidic Platform Fabricated with a ZnO Nanorod Template for Colorimetric Virus Detection

• DOI: 10.1021/acssensors.9b01927
• Publication Date: 2019-11-26T10:54:47Z
• Authors: Yiqiu Xia; Yizhu Chen; Yeming Tang; Gong Cheng; Xu Yu; Hongzhang He; Guohong Cao; Huaguang Lu; Zhiwen Liu; Si-Yang Zheng
• Keywords: Point of care; Nanorod; Point-of-Care Testing

Viruses pose serious infectious disease threats to humans and animals. To significantly decrease the mortality morbidity caused by virus infections, there is an urgent need of sensitive rapid point-of-care platforms for detection, especially in low-resource settings. Herein, we developed a smartphone-based platform highly selective detection avian influenza based on nanomaterial-enabled colorimetric detection. The 3D nanostructures, which serve as scaffold antibody conjugation capture virus, are made PDMS herringbone structures with ZnO nanorod template. After capture, on-chip gold nanoparticle-based reaction allows naked eyes limit 2.7 × 104 EID50/mL, one order magnitude better than that conventional fluorescence-based ELISA. Furthermore, smartphone imaging system data processing capability further improves limit, reaching down 8 103 EID50/mL. entire process can be completed 1.5 h. We envision this microfluidic integrated would provide fast, cheap, sensitive, user-friendly