Engineering a Controllable Targeted Protein Degradation System and a Derived OR-GATE-Type Inducible Gene Expression System in Synechococcus elongatus PCC 7942

• DOI: 10.1021/acssynbio.1c00226
• Publication Date: 2021-12-16T20:40:02Z
• Authors: Mingyi Zhang; Quan Luo; Huili Sun; Jacques Fritze; Guodong Luan; Xuefeng Lu
• Keywords: Synthetic Biology; Metabolic Engineering; Lac repressor; Protein Degradation

Cyanobacteria are important model organisms for exploring the mechanisms of photosynthesis and considered as promising microbial platforms photosynthetic biomanufacturing. The development efficient cyanobacteria cell factories requires convenient tools to dynamically regulate manipulate target proteins, modules, pathways. Targeted protein degradation is achieve rapid responses cellular metabolic networks artificial or environmental signals, there currently limited approaches induce in cyanobacteria. In this work, we developed an Escherichia coli sourced ssrA-tagging system strain, Synechococcus elongatus PCC 7942, inducible proteins. A modified version E. ssrA tag (ssrADAS) proved be immune native ClpXP while induced expression adaptor SspB resulted effective tagged Compared previously down-regulation approaches, ssrADAS–SspB-ClpXPEc facilitated smart proteins PCC7942 cells at different growth stages. Furthermore, when used LacI, repressor element LacO-LacI transcription regulation system, stringent gene was obtained based on OR-GATE type genetic circuit design. work expanded synthetic biology toolbox will facilitate success future dynamic engineering.