Engineering a Synthetic Pathway for Tyrosol Synthesis in Escherichia coli

• DOI: 10.1021/acssynbio.1c00517
• Publication Date: 2022-01-05T21:31:28Z
• Authors: Yumeng Lai; Haofeng Chen; Lingrui Liu; Bixia Fu; Peiling Wu; Wanrong Li; Junyan Hu; Jifeng Yuan
• Keywords: Tyrosol; Metabolic Engineering; Pseudomonas putida

Tyrosol is an aromatic compound with great value that widely used in the food and pharmaceutical industry. In this study, we reported a synthetic pathway for converting p-coumaric acid (p-CA) into tyrosol Escherichia coli. We found enzyme cascade comprising ferulic decarboxylase (FDC1) from Saccharomyces cerevisiae, styrene monooxygenase (SMO), oxide isomerase (SOI) Pseudomonas putida, phenylacetaldehyde reductase (PAR) Solanum lycopersicum could efficiently synthesize p-CA conversion rate over 90%. To further expand range of substrates, also introduced tyrosine ammonia-lyase (TAL) Flavobacterium johnsoniae to connect endogenous l-tyrosine metabolism. be produced glycerol, reaching 545.51 mg/L tyrosine-overproducing strain under shake flasks. summary, have established alternative routes synthesis (a potential lignin-derived biomass), glucose, glycerol.