Efficient Bacterial Genome Engineering throughout the Central Dogma Using the Dual-Selection Marker tetAOPT

• DOI: 10.1021/acssynbio.2c00345
• Publication Date: 2022-10-07T19:55:48Z
• Authors: Carolyn N. Bayer; Ana G. V. Sepulchro; Maja Rennig; Morten H.H. Nørholm
• Keywords: Genome Engineering; Synthetic Biology

Engineering of bacterial genomes is a fundamental craft in contemporary biotechnology. The ability to precisely edit chromosomes allows for the development cells with specific phenotypes metabolic engineering and creation minimized genomes. Genetic tools are needed select that underwent editing, dual-selection markers enable both positive negative selection highly useful. Here, we present an optimized easy-to-use version