Advances in Engineering Nucleotide Sugar Metabolism for Natural Product Glycosylation in Saccharomyces cerevisiae

• DOI: 10.1021/acssynbio.3c00737
• Publication Date: 2024-05-31T19:11:42Z
• Authors: Samantha A. Crowe; Yuzhong Liu; Xixi Zhao; Henrik V. Scheller; Jay D. Keasling
• Keywords: Nucleotide sugar; Metabolic Engineering

Glycosylation is a ubiquitous modification present across all of biology, affecting many things such as physicochemical properties, cellular recognition, subcellular localization, and immunogenicity. Nucleotide sugars are important precursors needed to study glycosylation produce glycosylated products. Saccharomyces cerevisiae potentially powerful platform for producing biomolecules, but it lacks nucleotide sugar diversity. metabolism complex, understanding how engineer will be necessary both access heterologous glycosylations found biology. This review overviews the potential challenges with engineering in yeast from salvage pathways that convert free their associated UDP-sugars de novo synthesis where interconverted through complex metabolic network governing feedback mechanisms. Finally, recent examples small molecules S. explored assessed.