Characterizing and Tailoring the Substrate Profile of a γ-Glutamyltransferase Variant

• DOI: 10.1021/acssynbio.4c00364
• Publication Date: 2024-08-12T23:51:25Z
• Authors: David Mueller; Remo Baettig; Tilmann Kuenzl; Emilio Rodríguez-...; Tania Michelle Ro...; Philippe Marlière; Sven Panke
• Keywords: Synthetic Biology

Xenobiology is an emerging field that focuses on the extension and redesign of biological systems through use laboratory-derived xenomolecules, which are molecules new to metabolism cell. Despite enormous potential using xenomolecules in living organisms, most noncanonical building blocks still need be supplied externally, often poor uptake into cells limits wider applicability. To improve cytosolic availability molecules, a synthetic transport system based portage was developed, interest "cargo" linked vector enables piggyback alkylsulfonate transporter (SsuABC)