Controlling Multicycle Replication of Live-Attenuated HIV-1 Using an Unnatural Genetic Switch

• DOI: 10.1021/acssynbio.6b00373
• Publication Date: 2017-01-21T01:06:35Z
• Authors: Zhe Yuan; Nanxi Wang; Guobin Kang; Wei Niu; Qingsheng Li; Jiantao Guo
• Keywords: Attenuated vaccine; Simian immunodeficiency virus; HIV vaccine; Replication

A safe and effective human immunodeficiency virus type 1 (HIV-1) vaccine is urgently needed, but remains elusive. While HIV-1 live-attenuated can provide potent protection as demonstrated in rhesus macaque-simian model, the potential pathogenic consequences associated with uncontrolled replication preclude such from clinical applications. We investigated a novel approach to address this problem by controlling through an unnatural genetic switch that was based on amber suppression strategy. Here we report construction of all-in-one mutants contain genomic copy system. This modification resulted viruses were capable multicycle vitro could be switched off using amino acid cue. stand-alone, replication-controllable attenuated represents important step toward generation efficacious vaccine. The strategy reported work adopted for development other vaccines.