Corynebacterium glutamicum Chassis C1*: Building and Testing a Novel Platform Host for Synthetic Biology and Industrial Biotechnology

• DOI: 10.1021/acssynbio.7b00261
• Publication Date: 2017-08-14T05:09:21Z
• Authors: Meike Baumgart; Simon Unthan; Ramona Kloß; Andreas Radek; Tino Polen; Niklas Tenhaef; Moritz Fabian Müller; Andreas Küberl; Daniel Siebert; Natalie Brühl; Kay Marin; Stephan Hans; Reinhard Krämer; Michael Bott; Jörn Kalinowski; Wolfgang Wiechert; Gerd Seibold; Julia Frunzke; Christian Rückert; Volker F. Wendisch; Stephan Noack
• Keywords: Corynebacterium glutamicum; Chassis; Synthetic Biology; Strain (injury)

Targeted top-down strategies for genome reduction are considered to have a high potential providing robust basic strains synthetic biology and industrial biotechnology. Recently, we created library of 26 genome-reduced Corynebacterium glutamicum carrying broad deletions in single gene clusters showing wild-type-like biological fitness. Here, proceeded with combinatorial these irrelevant two parallel orders, the resulting 28 was characterized under various environmental conditions. The final chassis strain C1* carries 13.4% (412 deleted genes) shows growth behavior defined medium d-glucose as carbon energy source. Moreover, proves be against several stresses (including oxygen limitation) long-term stability complex In addition novel prokaryotic strain, our results comprise large revised annotation list, which will valuable sources future systemic studies C. glutamicum.