Optogenetics Manipulation Enables Prevention of Biofilm Formation of Engineered Pseudomonas aeruginosa on Surfaces

• DOI: 10.1021/acssynbio.7b00273
• Publication Date: 2017-10-23T15:01:02Z
• Authors: Lu Pu; Shuai Yang; Aiguo Xia; Fan Jin
• Keywords: Synthetic Biology

Synthetic biologists have attempted to solve real-world problems, such as those of bacterial biofilms, that are involved in the pathogenesis many clinical infections and difficult eliminate. To address this, we employed a blue light responding system integrated it into chromosomes Pseudomonas aeruginosa. With making rational adaptions improvements light-activated system, provided robust convenient means spatiotemporally control gene expression manipulate biological processes with minimal perturbation P. It increased light-induced up 20-fold. Moreover, deliberately introduced functional protein PA2133 containing an EAL domain degrade c-di-GMP modified showed optimally engineered optogenetic tool inhibited formation aeruginosa biofilms through induction light, resulting much sparser thinner biofilms. Our approach establishes methodology for leveraging tools synthetic biology guide biofilm engineer patterns unprecedented spatiotemporal resolution. Furthermore, findings suggest may provide promising strategy could be applied fight