Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii

• DOI: 10.1021/acssynbio.8b00251
• Publication Date: 2018-08-31T03:36:36Z
• Authors: Pierre Crozet; Francisco J. Navarro; Felix Willmund; Payam Mehrshahi; Kamil Bakowski; Kyle J. Lauersen; Maria-Esther Pére...; Pascaline Auroy; Aleix Gorchs Rovira; Susana Sauret-Gueto; Justus Niemeyer; Benjamin Spaniol; Jasmine Theis; Raphael Trösch; Lisa-Desiree West...; Konstantinos Vavi...; Thomas Baier; Wolfgang Hübner; Felix de Carpentier; Mathieu Cassarini; Antoine Danon; Julien Henri; Christophe H. Mar...; Marcello de Mia; Kevin Sarkissian; David C. Baulcombe; Gilles Peltier; José-Luis Crespo; Olaf Kruse; Poul-Erik Jensen; Michael Schroda; Alison G. Smith; Stéphane D. Lemaire
• Keywords: Chlamydomonas reinhardtii; Synthetic Biology; Chlamydomonas; Chassis; Cloning (programming)

Microalgae are regarded as promising organisms to develop innovative concepts based on their photosynthetic capacity that offers more sustainable production than heterotrophic hosts. However, realize potential green cell factories, a major challenge is make microalgae easier engineer. A approach for rapid and predictable genetic manipulation use standardized synthetic biology tools workflows. To this end we have developed Modular Cloning toolkit the microalga Chlamydomonas reinhardtii. It Golden Gate cloning with standard syntax, comprises 119 openly distributed parts, most of which been functionally validated in several strains. contains promoters, UTRs, terminators, tags, reporters, antibiotic resistance genes, introns cloned various positions allow maximum modularity. The enables building engineered cells both fundamental research algal biotechnology. This work will next chassis biology.