Artificial Metalloglycoclusters: Compact Saccharide Shell to Induce High Lectin Affinity as Well as Strong Luminescence

• DOI: 10.1021/bc020026a
• Publication Date: 2003-07-16T04:35:08Z
• Authors: Teruaki Hasegawa; Takahiro Yonemura; Kazunori Matsuura; Kazukiyo Kobayashi
• Keywords: Affinities

Tris-bipyridine ferrous and ruthenium complexes carrying various saccharide appendages have been investigated to develop sensory systems for monitoring saccharide-binding phenomena. Ferrous O-glycoclusters having spacer moieties inserted between the complex core showed enhanced affinities lectins, but N-glycoclusters, in which saccharide-appendages are directly linked via amide linkage, had low lectin-affinities. Molecular dynamics calculation indicated that flexible densely packed clusters, contrast octahedrally fixed arrays of N-glycoclusters. Flexibility clusters is essential their affinity, probably induce conformational change fit recognition sites lectins. According these insights, designed as luminescence biosensors. The α-manno exhibited excellent (ICmin = 9.0 × 10-8 M) concanavalin A (ConA). It suggested from analysis mannoclusters can be properly site ConA. binding was enthalpicaly driven (ΔH° −21.8 kcal/mol). This behavior quite similar 1−3/1−6 trimannoside They strongly amplified (Φem 0.15), intensities were changed (∼40%) upon specific glycoclusters a suitable system