Lipid Profile Altered in Phenanthrene Exposed Zebrafish Embryos with Implications for Neurological Development and Early Life Nutritional Status

• DOI: 10.1021/envhealth.3c00002
• Publication Date: 2023-05-30T12:05:04Z
• Authors: Victoria McGruer; Anil Bhatia; Jason T. Magnuson; Daniel Schlenk
• Keywords: Yolk

Lecithotrophic fish embryos rely on finite maternally deposited yolk resources for early development. Toxicant exposure can disrupt the uptake of with consequences In this study, we investigate impacts altered utilization using cardiotoxic compound phenanthrene. Zebrafish were exposed to a concentration phenanthrene beginning at 6 hpf (hours post-fertilization) until maximum 72 hpf. Embryos stained Oil Red O visualize neutral lipids. We then used nontargeted approach profile lipids in 24 and after treatment. To assess changes lipid movement within embryo, sac was dissected from body analyzed separately Overall, total metabolites significantly reduced sac, staining embryo This result is consistent significant reductions triglycerides both yolk, indicating limited contribution impaired cardiac function mobilization dose tested. Additionally, lysophosphatidylcholines lysophosphatidylethanolamines increased body. Bioinformatic pathway analysis indicated that these lysophospholipids could be linked disease model associated inflammation neuron demyelination previously observed injuries neuronal eye development larvae.