Developmental Neurobiology Implications from Fabrication and Analysis of Hippocampal Neuronal Networks on Patterned Silane-Modified Surfaces

• DOI: 10.1021/ja973669n
• Publication Date: 2002-07-26T05:23:52Z
• Authors: M. S. Ravenscroft; K. E. Bateman; K. M. Shaffer; H. M. Schessler; D. R. Jung; T. W. Schneider; C. B. Montgomery; T. L. Custer; A. E. Schaffner; Q. Y. Liu; Y. X. Li; J. L. Barker; J. J. Hickman
• Keywords: Neurite

We have determined the parameters necessary to fabricate reproducible neuronal patterns which we are using begin studying fundamental issues in developmental neurobiology. The addition of a beam homogenizer, as well new surface preparation, has enabled routine production reproducible, high-resolution (2−20 μm) organosilane patterns. effects preparation and dosage were monitored X-ray photoelectron spectroscopy (XPS) proof patterning is provided by imaging XPS. also report guidance adhesion neurite outgrowth creation reproducibly defined circuits embryonic (E18−19) rat hippocampal neurons these patterned surfaces vitro. achieved >50% rate pattern formation, at times approaches 90%. address issue how geometric cues might be used affect cell-to-cell communication preliminary results on synaptic development dual patch-clamp electrophysiology. emergence both spontaneous evoked activity for unpatterned (control) cultures. indicate intriguing possibility that geometry itself may modulating or trophic factor cell development.