Rapid and Specific Imaging of Extracellular Signaling Molecule Adenosine Triphosphate with a Self-Phosphorylating DNAzyme

• DOI: 10.1021/jacs.1c04925
• Publication Date: 2021-08-20T15:02:42Z
• Authors: Dan Zhao; Dingran Chang; Qiang Zhang; Yangyang Chang; Bo Liu; Changsen Sun; Zhonping Li; Chuan Dong; Meng Liu; Yingfu Li
• Keywords: Adenosine triphosphate; Cell Signaling; Substrate-level phosphorylation; Deoxyribozyme

Adenosine 5'-triphosphate (ATP) is a central extracellular signaling agent involved in various physiological and pathological processes. However, precise measurements of the temporal spatial components ATP dynamics are lacking due primarily to limitations available methods for detection. Here, we report on first effort design self-phosphorylating DNAzyme (SPDz) sensor fluorescence imaging ATP. In response ATP, SPDz sensors exhibit subsecond kinetics, extremely high specificity, micromolar affinities. particular, demonstrate cell-surface-anchored both stress-induced endogenous release astrocytes mechanical stimulation-evoked at single-cell level. We also validated their utility visualizing rapid dynamic properties upon electrical stimulation astrocytes. Thus, robust tools monitoring underlying diverse cellular