Förster Resonance Energy Transfer Switchable Self-Assembled Micellar Nanoprobe: Ratiometric Fluorescent Trapping of Endogenous H2S Generation via Fluvastatin-Stimulated Upregulation

• DOI: 10.1021/jacs.5b03248
• Publication Date: 2015-06-12T21:52:08Z
• Authors: Chunchang Zhao; Xiuli Zhang; Kaibin Li; Shaojia Zhu; Zhiqian Guo; Lili Zhang; Feiyi Wang; Qiang Fei; Sihang Luo; Ping Shi; He Tian; Wei-Hong Zhu
• Keywords: Cyanine

H2S produced in small amounts by mammalian cells has been identified mediating biological signaling functions. However, the situ trapping of endogenous generation is still handicapped a lack straightforward methods with high selectivity and fast response. Here, we encapsulate semi-cyanine-BODIPY hybrid dye (BODInD-Cl) its complementary energy donor (BODIPY1) into hydrophobic interior an amphiphilic copolymer (mPEG-DSPE), especially for building up ratiometric fluorescent nanoprobe extraordinarily A remarkable red-shift absorption band gap 200 nm response can efficiently switch off Förster resonance transfer (FRET) from BODIPY1 to BODInD-Cl, subsequently recovering fluorescence. Impressively, both hydrophobicity supramolecular micelles electron-withdrawing nature indolium unit BODInD-Cl sharply increase aromatic nucleophilic substitution H2S. The strategy based on unique self-assembled micellar aggregate NanoBODIPY achieves extremely response, enabling imaging production mapping physiological pathological consequences. Moreover, renders assembly biocompatible soluble aqueous solution. established FRET-switchable macromolecular envelope around enables cellular uptake, makes breakthrough within raw264.7 macrophages upon stimulation fluvastatin. This study manifests that cystathione γ-lyase (CSE) upregulation contributes fluvastatin-stimulated macrophages, along correlation between CSE/H2S activating Akt pathway.