Light-Responsive and pH-Responsive DNA Microcapsules for Controlled Release of Loads

• DOI: 10.1021/jacs.6b04773
• Publication Date: 2016-06-16T19:33:34Z
• Authors: Fujian Huang; Wei-Ching Liao; Yang Sung Sohn; Rachel Nechushtai; Chun-Hua Lu; Itamar Willner
• Keywords: Allylamine; Doxorubicin Hydrochloride; Cleavage (geology); Hydrochloride

A method to assemble light-responsive or pH-responsive microcapsules loaded with different loads (tetramethylrhodamine-modified dextran, TMR-D; microperoxidase-11, MP-11; CdSe/ZnS quantum dots; doxorubicin-modified DOX-D) is described. The based on the layer-by-layer deposition of sequence-specific nucleic acids poly(allylamine hydrochloride)-functionalized CaCO3 core microparticles, loads, that after dissolution particles EDTA yields stimuli-responsive include respective loads. are composed photocleavable o-nitrobenzyl-phosphate-modified DNA shells, and made a cytosine-rich layer cross-linked by acid bridges. Irradiating o-nitrobenzyl phosphate-functionalized microcapsules, λ = 365 nm, subjecting pH 5.0, results in cleavage microcapsule shells release Preliminary studies address cytotoxicity DOX-D-loaded toward MDA-MB-231 breast cancer cells normal MCF-10A epithelial cells. Selective demonstrated.