Enzyme-Responsive Procarriers Capable of Transporting Chloride Ions across Lipid and Cellular Membranes

• DOI: 10.1021/jacs.6b10592
• Publication Date: 2016-11-16T12:07:41Z
• Authors: Ye Rin Choi; Bom Lee; Jinhong Park; Wan Namkung; Kyu-Sung Jeong
• Keywords: Ions; Chlorides; Molecular Structure; Cell Membrane; Esterases; Hydrophobic and Hydrophilic Interactions; 01 natural sciences; DNA Glycosylases; 0104 chemical sciences

Adopting the concept of procarrier for first time, we demonstrated controlled transport chloride ions across lipid and cellular membranes. Procarriers containing highly hydrophilic appendages were initially inactive due to lack their partitioning into lipophilic membranes but activated in presence specific enzymes that able hydrolyze off generate an active carrier under conditions. Namely, with ester-bond-linked appendage was most by esterase (PLE) at pH = 7.4, whereas a glycosyl-bond-linked only glycosylase (AOG) slightly acidic conditions (pH 5.5-6). In addition controlling transport, greatly increase water solubility procarrier, which may improve deliverability hydrophobic plasma membrane.