Incorporation of a Disposable ESI Emitter into Inline Cartridge Extraction Mass Spectrometry Improves Throughput and Spectra Stability

• DOI: 10.1021/jasms.2c00207
• Publication Date: 2022-12-19T22:29:07Z
• Authors: Denis S. Bormotov; Vasily A. Eliferov; Olga V. Peregudova; Denis S. Zavorotnyuk; Konstantin V. Boc...; Stanislav I. Pekov; Anatoly A. Sorokin; Eugene N. Nikolaev; Igor A. Popov
• Keywords: 0301 basic medicine; Spectrometry, Mass, Electrospray Ionization; 03 medical and health sciences; Humans; Reproducibility of Results

Rapid and reliable methods for detecting tumor margins are crucial for neuro-oncology. Several mass spectrometry-based methods have been recently proposed to address this problem. Inline Cartridge Extraction (ICE) demonstrates the potential for clinical application, based on ex-vivo analysis of dissected tissues, but requires time-consuming steps to avoid cross-contamination. In this work, a method of incorporating a disposable electrospray emitter into the ICE cartridge by PEEK sleeves melting is developed. It reduces total analysis time and improves throughput. The proposed setup also improves the robustness of the ICE molecular profiling as demonstrated with human glial tumor samples in that stability and reproducibility of the spectra were increased.