Quantitative Detection of Glyphosate by Simultaneous Analysis of UV Spectroscopy and Fluorescence Using DNA-Labeled Gold Nanoparticles

• DOI: 10.1021/jf102784t
• Publication Date: 2010-11-03T13:35:33Z
• Authors: Hee Uk Lee; Hyun Yong Shin; Jin Young Lee; Yoon Seok Song; ChulHwan Park; Seung Wook Kim
• Keywords: Quantitative Analysis

A sandwich-type immunosensor composed of antigen−double target/probe DNA-coated gold nanoparticles (NPs) was developed for the measurement fluorescence intensity and quantitative analysis single-stranded DNA based on concentration free glyphosate. The reaction between DNA−gold NPs immobilized antibody substrate carried out 2 h. results antigen−antibody were measured basis obtained from comparison with antigens at concentrations 0.01−100 μg mL−1 detection NPs. For glyphosate(0.01−100 mL−1), response also revealed same range glyphosate using detection.