Caffeic Acid Phenethyl Ester, a Major Component of Propolis, Suppresses High Fat Diet-Induced Obesity through Inhibiting Adipogenesis at the Mitotic Clonal Expansion Stage

• DOI: 10.1021/jf405088f
• Publication Date: 2014-03-10T20:52:53Z
• Authors: Seung Ho Shin; Sang Gwon Seo; Soyun Min; Hee Yang; Eunjung Lee; Joe Eun Son; Jung Yeon Kwon; Shuhua Yue; Min-Yu Chung; Kee-Hong Kim; Ji-Xin Cheng; Hyong Joo Lee; Ki Won Lee
• Keywords: Propolis; 3T3-L1

In the present study, we aimed to investigate antiobesity effect of CAPE in vivo, and mechanism by which regulates body weight vitro. To confirm mice were fed with a high fat diet (HFD) different concentrations for 5 weeks. significantly reduced gain epididymal mass obese HFD. accordance vivo results, Oil red O staining results showed that suppressed MDI-induced adipogenesis 3T3-L1 preadipocytes. FACS analysis delayed MDI-stimulated cell cycle progression, thereby contributing inhibit mitotic clonal expansion (MCE), is prerequisite step adipogenesis. Also, regulated expression cyclin D1 phosphorylation ERK Akt, are upstream D1. These suggest exerts an presumably through inhibiting at early stage