Characterization of Protein Clusters of Diverse Magnetic Nanoparticles and Their Dynamic Interactions with Human Cells

• DOI: 10.1021/jp809493t
• Publication Date: 2009-04-02T11:02:28Z
• Authors: Qingxin Mu; Zhenwei Li; Xi Li; Sanjay R. Mishra; Bin Zhang; Zhikun Si; Lei Yang; Wei Jiang; Bing Yan
• Keywords: Protein Adsorption; Iron oxide nanoparticles; Surface charge

Although nanoparticle/protein binding and the cytotoxicity of nanoparticles have been separately reported, there has no study linking nature clusters to cell uptake dynamic cellular responses. We report here that water-soluble iron oxide-based magnetic (MNPs) with different sizes surface chemistry bind serum proteins in terms protein identity quantity without changing secondary structures. Carboxylated MNPs (and aminated one smaller MNPs) resulted higher cytotoxicity, PEG coating reduced both cytotoxicity. Smaller (especially carboxylated one) more proteins, are much less taken up by cells as compared larger particles, yet elicit cytotoxic Besides intrinsic effects size charge MNPs, MNPs/protein were also attributed adsorbed rather than binding-induced new epitopes on proteins.