Formation, Spectroscopic Characterization, and Application of Sulfhydryl-Terminated Alkanethiol Monolayers for the Chemical Attachment of DNA onto Gold Surfaces

• DOI: 10.1021/la001064q
• Publication Date: 2002-07-26T04:56:38Z
• Authors: Emily A. Smith; Matt J. Wanat; Yufei Cheng; Sérgio V. P. Barr...; Anthony G. Frutos; Robert M. Corn
• Keywords: Characterization; Self-assembled monolayer

A novel surface modification procedure for the creation of sulfhydryl-terminated alkanethiol monolayers that can be used attachment biomolecules onto gold surfaces is described. self-assembled monolayer amine-terminated 11-mercaptoundecylamine (MUAM) reacted with heterobifunctional cross-linker N-succinimidyl S-acetylthiopropionate (SATP) in order to create a protected monolayer. This then deprotected an alkaline solution active sulfhydryl surface. Compounds have been modified contain maleimide moiety easily attached sulfhydryl-derivatized In second strategy, 2,2'-dipyridyl disulfide form bonds on These are thiol−disulfide exchange reaction free sulfhydryls attach biomolecules, such as thiol-modified DNA or cysteine-containing polypeptides, contrast maleimide-attached monolayers, disulfide-immobilized species cleaved presence dithiothreitol (DTT) regenerate Polarization modulation FTIR reflection−absorption spectroscopy (PM-FTIRRAS) has characterize these reactions, and fluorescence "wash off" measurements provided estimate 1.5 × 1012 molecules/cm2 coverage immobilized using reaction. Surface plasmon resonance (SPR) imaging were employed monitor situ hybridization arrays fabricated this immobilization