Fluorescence Imaging of the Oxidative Desorption of a BODIPY-Alkyl-Thiol Monolayer Coated Au Bead

• DOI: 10.1021/la800233c
• Publication Date: 2008-06-21T08:00:33Z
• Authors: Amanda Musgrove; Arnold Kell; Dan Bizzotto
• Keywords: BODIPY

The reductive and oxidative desorption of a BODIPY labeled alkylthiol self-assembled monolayer (SAM) on Au was studied using electrochemical methods coupled with fluorescence microscopy image analysis procedures to monitor the removal adsorbed layer. Two SAMs were formed two lengths alkyl chain (C10 C16). fluorescent moiety used is known form dimers which through donor−acceptor energy transfer results in red-shifted fluorescence. Fluorescence from monomer dimer study nature desorbed molecules during cyclic step changes potential. observed occur over small potential window (0.15 V) signified by an increase capacitance Oxidative readsorption also decrease lack total Removal occurred at positive potentials broad range near oxidation bare Au. resulting showed that remained electrode surface not dispersed 20 s waiting time. rate change for much slower than desorption. Comparing intensities indicated as dimer, rather forming monomers surface. can only be excited suggesting must close proximity aggregates electrode. yield longer always lower presumably due its decreased solubility interfacial region more efficient quenching. process significantly etched or roughened coupling thiol oxide formation