Design of Novel BSA/Hyaluronic Acid Nanodispersions for Transdermal Pharma Purposes

• DOI: 10.1021/mp400657g
• Publication Date: 2014-03-20T22:09:34Z
• Authors: Madalena Martins; Nuno G. Azoia; Ulyana Shimanovich; Teresa Matamá; Andreia C. Gomes; Carla Silva; Artur Cavaco-Paulo
• Keywords: 0303 health sciences; Science & Technology; Nanodispersion; Hyaluronic acid; Transdermal transport; Serum Albumin, Bovine; Fibroblasts; Skin penetration; Cell Line; Nanostructures; 3. Good health; 03 medical and health sciences; Bovine serum albumin; Drug Delivery Systems; Animals; Humans; Cattle; Hyaluronic Acid; Skin

A novel transdermal hyaluronic acid (HA) conjugated with bovine serum albumin (BSA) was developed in the form of solid-in-oil (S/O) nanodispersion (129.7 nm mean diameter). Ex vivo skin penetration analysis by fluorescence and confocal observation of histological skin sections revealed the ability of BSA/HA nanodispersions to cross the stratum corneum and penetrate into the dermis. Furthermore, no significant toxicity was found in fibroblast and keratinocyte cells in vitro. These results proved the potential of the developed nanodispersion for transdermal delivery of hyaluronic acid constituting a high value to biopharmaceutical and cosmetics industries.