Azide Phosphoramidite in Direct Synthesis of Azide-Modified Oligonucleotides

• DOI: 10.1021/ol502155g
• Publication Date: 2014-08-26T12:13:39Z
• Authors: Maksim A. Fomich; Maksim V. Kvach; Maksim J. Navakouski; Christoph Weise; Alexander V. Bara...; Vladimir A. Korshun; Vadim V. Shmanai
• Keywords: Phosphoramidite; Oligonucleotide synthesis

Azide and phosphoramidite functions were found to be compatible within one molecule stable for months in solution kept frozen at −20 °C. An azide-carrying was used direct introduction of multiple azide modifications into synthetic oligonucleotides. A series azide-containing oligonucleotides modified further using click reactions with alkynes.