Effects of Intra- and Post-Operative Ischemia on the Metabolic Profile of Clinical Liver Tissue Specimens Monitored by NMR

• DOI: 10.1021/pr400702d
• Publication Date: 2013-10-14T11:35:51Z
• Authors: Stefano Cacciatore; Xiaoyu Hu; Christian Viertler; Marcel Kap; Gerwin A. Bernhardt; Hans-Jörg Mischinger; Peter Riegman; Kurt Zatloukal; Claudio Luchinat; Paola Turano
• Keywords: Magic angle spinning

Metabolomic profiles of tissues could greatly contribute to advancements in personalized medicine but are influenced by differences adopted preanalytical procedures; nonhomogeneous pre- and post-excision ischemia times potential sources variability. In this study, we monitored the impact on metabolic profiles, acquired with high-resolution magic-angle-spinning (1)H NMR, 162 human liver samples collected during up 6 h after routine surgery. The changed significantly as a function intraoperative warm (WI) postresection cold (CI) time, significant variations concentration same 16 metabolites. Therefore, tight control phase is essential for reliable metabolomic analyses diseases. NMR provide "fingerprint" have predictive value: best-performing models found discriminate extreme time points CI (0' vs 360 ') training set cross-validation accuracy ~90%; validation cohort can short (≤60') from long (≥180') an ~80%. For WI, corresponding figures 95.6 92%, respectively. might become tool tissue quality biobanks.