Discovery of Human sORF-Encoded Polypeptides (SEPs) in Cell Lines and Tissue

• DOI: 10.1021/pr401280w
• Publication Date: 2014-02-03T22:36:47Z
• Authors: Jiao Ma; Carl C. Ward; Irwin Jungreis; Sarah A. Slavoff; Adam G. Schwaid; John Neveu; Bogdan A. Budnik; Manolis Kellis; Alan Saghatelian
• Keywords: Human proteome project; Proteome; Proteogenomics; Human cell; K562 cells

The existence of nonannotated protein-coding human short open reading frames (sORFs) has been revealed through the direct detection their sORF-encoded polypeptide (SEP) products. discovery novel SEPs increases size genome and proteome provides insights into molecular biology mammalian cells, such as prevalent usage non-AUG start codons. Through modifications existing SEP-discovery workflow, we discover an additional 195 in K562 cells extend this methodology to identify cell lines tissue for a final tally 237 new SEPs. These results continue expand demonstrate that are ubiquitous class polypeptides require further investigation.