One Primer To Rule Them All: Universal Primer That Adds BBa_B0034 Ribosomal Binding Site to Any Coding Standard 10 BioBrick

• DOI: 10.1021/sb500047r
• Publication Date: 2014-12-19T05:03:50Z
• Authors: Anton V. Bryksin; Haylee N. Bachman; Spencer W. Cooper; Tilak Balavijayan; Rachael M. Blacks...; Haoli Du; Jackson P. Jenkins; Casey L. Haynes; Jessica L. Siemer; Vincent F. Fiore; Thomas H. Barker
• Keywords: Primer (cosmetics)

Here, we present a universal, simple, efficient, and reliable way to add small BioBrick parts any via PCR that is compatible with assembly standard 10. As proof of principle, have designed universal primer, rbs_B0034, contains ribosomal binding site (RBS; BBa_B0034) can be used in amplify coding starts ATG. We performed test PCRs rbs_B0034 on 31 different targets found it 93.6% efficient. Moreover, when supplemented complementary addition RBS accomplished whole plasmid site-directed mutagenesis, thus reducing the time required for further composite parts. The described method brings simplicity parts, such as regulatory elements existing BioBricks. final product indistinguishable from or 3A assembly.