Combinatorial Assembly of Large Biochemical Pathways into Yeast Chromosomes for Improved Production of Value-added Compounds

• DOI: 10.1021/sb500079f
• Publication Date: 2014-05-15T13:10:42Z
• Authors: Jifeng Yuan; Chi Bun Ching
• Keywords: Synthetic Biology; Value (mathematics); Metabolic Engineering

Saccharomyces cerevisiae as a eukaryotic organism is particularly suitable microbial cell factory because it has interesting features such membrane environments for supporting membrane-associated enzymes and its capability post-translational modifications of from plants. However, S. does not readily express polycistronic transcriptional units, which represents significant challenge constructing large biochemical pathways in budding yeast. In the present study, we developed novel approach rapid construction into yeast chromosomes. Our takes advantage antibiotic selection combinatorial assembly δ-sites retrotransposon elements As proof-of-principle, five-gene isobutanol pathway an eight-gene mevalonate were successfully assembled chromosomes one-step fashion. To our knowledge, this first report to exploit δ-integration coupled with We envision new could serve generalized technique yeast-a method that would be interest synthetic biology community.