Phenols and antioxidative status of Raphanus sativus grown in copper excess

• DOI: 10.1034/j.1399-3054.2003.00068.x
• Publication Date: 2004-12-28T15:14:02Z
• Authors: Cristina Sgherri; Elena Cosi; Flavia Navari‐Izzo
• Keywords: Raphanus; Phytochelatin

Raphanus sativus L. cv. Rimbo was grown for 10 days after emergence in hydroponic culture containing 0.12 (control), 5, and 15 µ M copper. The seeds were germinated the presence of copper solution. Cu contents increased with treatment both shoots roots, maintaining roots a value eight‐ to ten‐fold higher than shoots. With underwent growth inhibition an increase percentage dry weight. Membrane damage lipid peroxidation glutathione oxidized as concentration increased, indicating acceleration oxidative processes. Control had high reduced low phytochelatin‐SH whereas showed opposite pattern, suggesting utilization phytochelatin synthesis. In parts content reached maximum at 5 then decreased, reaching control five‐fold main phenolic acids represented R. chlorogenic, vanillic, caffeic, siringic, p‐coumaric ferulic least gallic, protocatechuic p‐hydroxybenzoic acids. well total ascorbate intensification treatment. Notwithstanding these changes, remained 35% roots. thus able replenish reducing equivalents phenoxyl radicals explaining compounds.