Nicotine effects on polymorphonuclear cell apoptosis and lipopolysaccharide‐induced monocyte functions. A possible role in periodontal disease?

• DOI: 10.1034/j.1600-0765.2001.00301.x
• Publication Date: 2003-03-12T08:54:56Z
• Authors: M. A. Mariggiò; L. Guida; A. Laforgia; R. Santacroce; E. Curci; P. Montemurro; R. Fumarulo
• Keywords: Monocyte; efferocytosis

Apoptosis provides a mechanism for clearance of unwanted cells in variety situations which programmed or physiological cell death occurs; but the premature defensive could promote infection, inflammation and concomitant disease. We detected high values apoptosis polymorphonuclear (PMN) elicited from crevicular sulci smokers affected by adult periodontitis. To learn more about effects nicotine on periodontal environment, we studied its ability to modulate two phagocytic lines, PMN mononuclear cells, are continuously recruited gingival vessels prevent control plaque extension. Brief exposure concentrations ranging 0.01 0.3% shortened, dose-dependent relationship, lag culture time required observe at fluorescent microscopy morphological traces apoptosis. These observations were confirmed specific tools apoptosis: DNA fragmentation gel electrophoresis expression apoptosis-signaling receptor Fas/Apo-1. The apoptotic effect excited these first line may be an important feature pathogenesis As leukocytes, was unable induce modifications observed up 72 h time, drug inhibited IL-1beta release procoagulant activity (PCA) expression. conflicting role played lipopolysaccharide (LPS)-induced monocyte functions inflammatory process is further intrigue compromises oral health.