Isolation of cDNA clones encoding the 20K T3 glycoprotein of human T-cell receptor complex

• DOI: 10.1038/312413a0
• Publication Date: 2004-11-18T02:01:38Z
• Authors: Peter van den Elsen; Beth-Ann Shepley; Jannie Borst; John E. Coligan; Alexander F. Markham; Stuart Orkin; Cox Terhorst
• Keywords: Isolation

The complete amino acid sequence of one of the polypeptide chains of the human T3/T-cell receptor complex, the T3 glycoprotein (T3-delta-chain) of relative molecular mass 20,000, was deduced from cDNA clones derived from HPB-ALL cells and a human T-cell clone. Inspection of the 171-amino acid sequence reveals a signal peptide, a 79-amino acid extracellular domain, one transmembrane region and a 44-amino acid intracellular domain which show no sequence homology with members of the T-cell receptor/immunoglobulin/MHC multi-gene family. The T3 delta-chain is coded for by a single-copy gene the expression of which is restricted to T lymphocytes in humans and mice.