Requirement for hsp70 in the mitochondrial matrix for translocation and folding of precursor proteins

• DOI: 10.1038/348137a0
• Publication Date: 2003-06-12T22:56:06Z
• Authors: Pil-Jung Kang; Joachim Ostermann; Jeffery Shilling; Walter Neupert; Elizabeth A. Craig; Nikolaus Pfanner
• Keywords: 0303 health sciences; Protein Conformation; DNA Mutational Analysis; Protozoan Proteins; Biological Transport; Cell Compartmentation; Mitochondria; Fungal Proteins; 03 medical and health sciences; Mutation; HSP70 Heat-Shock Proteins; Cloning, Molecular; Protein Precursors; Protein Processing, Post-Translational; Heat-Shock Proteins

By analysis of a temperature-sensitive yeast mutant, a heat-shock protein in the matrix of mitochondria, mitochondrial hsp70 (Ssc1p), is found to be involved both in translocation of nuclear-encoded precursor proteins across the mitochondrial membranes and in (re)folding of imported proteins in the matrix.