Bone marrow-derived mesenchymal stem cells ameliorate chronic high glucose-induced β-cell injury through modulation of autophagy

• DOI: 10.1038/cddis.2015.230
• Publication Date: 2015-09-17T12:44:02Z
• Authors: K Zhao; H Hao; J Liu; C Tong; Y Cheng; Z Xie; L Zang; Y Mu; W Han
• Keywords: Viability assay

Abstract Chronic hyperglycemia causes a progressive decrease of β -cell function and mass in type 2 diabetic patients. Growing evidence suggests that augment autophagy may be an effective approach to protect cells against various extra-/intracellular stimuli. In this study, we thus investigated whether bone marrow-derived mesenchymal stem (BM-MSCs) could ameliorate chronic high glucose (HG)-induced injury through modulation autophagy. Prolonged exposure HG decreased cell viability, increased apoptosis impaired basal insulin secretion glucose-stimulated INS-1 cells, but BM-MSC treatment significantly alleviated these glucotoxic alternations. addition, western blotting displayed upregulated expression Beclin1 LC3-II co-cultured with BM-MSCs. Results from immunofluorescence staining transmission electronic microscope analysis also revealed BM-MSCs promoted autophagosomes autolysosomes formation HG-treated cells. However, it should noted inhibition diminished the protective effects on suggesting improvement survival induced by was mediated Furthermore, our results showed improved mitochondrial reduced reactive oxygen species production largely owing autophagic clearance mitochondria. vivo study performed rats diabetes (T2D). infusion not only ameliorated hyperglycemia, restoration pancreatic T2D rats. Meanwhile, LAMP2 enhanced autolysosomes, combined number granules. These findings together indicated HG-induced vitro . This unveiled novel as ideal strategy enhance for mellitus.