Processing Pisum sativum seed storage protein precursors in vitro

• DOI: 10.1038/cr.1990.15
• Publication Date: 2012-03-20T12:09:29Z
• Authors: Lijun Yang; C Domoney; R Casey; TC Hall
• Keywords: Legumin; Vicilin; Cleavage (geology)

The profile of polypeptides separated by SDS-PAGE from seed of major crop species such as pea (Pisum sativum) is complex, resulting from cleavage (processing) of precursors expressed from multiple copies of genes encoding vicilin and legumin, the major storage globulins. Translation in vitro of mRNAs hybrid-selected from mid-maturation pea seed RNAs by defined vicilin and legumin cDNA clones provided precursor molesules that were cleaved in vitro by a cell-free protease extract obtained from similar stage seed; the derived polypeptides were of comparable sizes be those observed in rivo. The feasibility of transcribing mRNA in vitro from a cDNA clone and cleavage in vitro of the derived translation products was established for a legumin clone, providing a method for determining polypeptide products of an expressed sequence. This approach will also be useful for characterising cleavage site requirements since modifications an readily be introduced at the DNA level.