Robust In Vivo Transduction of Nervous System and Neural Stem Cells by Early Gestational Intra Amniotic Gene Transfer Using Lentiviral Vector

• DOI: 10.1038/mt.2010.125
• Publication Date: 2010-06-22T14:13:19Z
• Authors: David H Stitelman; Masayuki Endo; Archana Bora; Nidal Muvarak; Philip W Zoltick; Alan W Flake; Timothy R Brazelton
• Keywords: Transduction (biophysics); Gene transfer

Presently, in vivo methods to efficiently and broadly transduce all major cell types throughout both the central (CNS) peripheral adult nervous system (PNS) are lacking. In this study, we hypothesized that during early fetal development neural populations, including stem cells (NSCs), may be accessible for gene transfer via open groove. To test hypothesis, injected lentiviral vectors encoding a green fluorescent protein (GFP) marker into murine amniotic cavity at embryonic day 8. This method (i) stably transduced entire least 80% of lifespan mice, (ii) types, (iii) NSCs subventricular zone (SVZ) subgranular zones (SGZs). simple approach has broad applications study function have future clinical treatment genetic disorders system.