Targeted Expression of miR-34a Using the T-VISA System Suppresses Breast Cancer Cell Growth and Invasion

• DOI: 10.1038/mt.2012.201
• Publication Date: 2012-10-02T16:11:45Z
• Authors: Laisheng Li; Xinhua Xie; Jinmei Luo; Min Liu; Shaoyan Xi; Jiaoli Guo; Yanan Kong; Minqing Wu; Jie Gao; Zeming Xie; Jun Tang; Xi Wang; Weidong Wei; Mingtian Yang; Mien-Chie Hung; Xiaoming Xie
• Keywords: Pharmacology; 0301 basic medicine; Mice, Inbred BALB C; Blotting, Western; Breast Neoplasms; Flow Cytometry; Real-Time Polymerase Chain Reaction; Immunohistochemistry; 3. Good health; Mice; MicroRNAs; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Drug Discovery; Genetics; Molecular Medicine; Animals; Humans; Female; Molecular Biology

Recurrence and metastasis result in a poor prognosis for breast cancer patients. Recent studies have demonstrated that microRNAs (miRNAs) play vital roles the development of cancer. In this study, we investigated therapeutic potential miR-34a We found is downregulated cell lines tissues, compared with normal adjacent nontumor respectively. To explore miR-34a, designed targeted expression plasmid (T-VISA-miR-34a) using T-VISA system, evaluated its antitumor effects, efficacy, mechanism action, systemic toxicity. T-VISA-miR-34a induced robust, persistent dramatically suppressed growth, migration, invasion vitro by downregulating protein levels target genes E2F3, CD44, SIRT1. an orthotopic mouse model cancer, intravenous injection T-VISA-miR-34a:liposomal complex nanoparticles significantly inhibited tumor prolonged survival, did not induce conclusion, lead to specific overexpression cells potent effects vivo. may provide potentially useful, specific, safe-targeted approach