Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome

• DOI: 10.1038/nbt.2624
• Publication Date: 2013-07-21T20:06:09Z
• Authors: Nathan E Lewis; Xin Liu; Yuxiang Li; Harish Nagarajan; George Yerganian; Edward O'Brien; Aarash Bordbar; Anne M Roth; Jeffrey Rosenbloom; Chao Bian; Min Xie; Wenbin Chen; Ning Li; Deniz Baycin-Hizal; Haythem Latif; Jochen Forster; Michael J Betenbaugh; Iman Famili; Xun Xu; Jun Wang; Bernhard O Palsson
• Keywords: Cricetulus; Chinese hamster

Genome sequences of the Chinese hamster and six ovary cell lines provide a resource to aid in improving production recombinant proteins. (CHO) cells, first isolated 1957, are preferred host for many therapeutic Although genetic heterogeneity among CHO has been well documented, systematic, nucleotide-resolution characterization their genotypic differences stymied by lack unifying genomic cells. Here we report 2.4-Gb draft genome sequence female hamster, Cricetulus griseus, harboring 24,044 genes. We also resequenced analyzed genomes from CHO-K1, DG44 CHO-S lineages. This analysis identified genes missing different lines, detected >3.7 million single-nucleotide polymorphisms (SNPs), 551,240 indels 7,063 copy number variations. Many mutations located with functions relevant bioprocessing, such as apoptosis. The details this diversity highlight value reference upon which cells can be studied engineered protein production.