Synthesizing topological structures containing RNA

• DOI: 10.1038/ncomms14936
• Publication Date: 2017-03-31T09:59:38Z
• Authors: Di Liu; Yaming Shao; Gang Chen; Yuk-Ching Tse-Dinh; Joseph A. Piccirilli; Yossi Weizmann
• Keywords: Transcription

Though knotting and entanglement have been observed in DNA proteins, their existence RNA remains an enigma. Synthetic topological structures are significant for understanding the physical biological properties pertaining to topology, these turn could facilitate identifying naturally occurring topologically nontrivial molecules. Here we show that containing single-stranded (ssRNA) free of strong base pairing interactions can be created either by configuring RNA-DNA hybrid four-way junctions or template-directed synthesis with a (ssDNA) structure. By using constructed ssRNA knot as highly sensitive probe, find Escherichia coli topoisomerase I has low activity R173A point mutation abolishes unknotting ssRNA, but not ssDNA. Furthermore, discover inhibition reverse transcription (RT) obtain different RT-PCR patterns circle same sequence.