Single-cell RNA-seq enables comprehensive tumour and immune cell profiling in primary breast cancer

• DOI: 10.1038/ncomms15081
• Publication Date: 2017-05-05T10:09:55Z
• Authors: Woosung Chung; Hye Hyeon Eum; Hae-Ock Lee; Kyung-Min Lee; Han-Byoel Lee; Kyu-Tae Kim; Han Suk Ryu; Sangmin Kim; Jeong Eon Lee; Yeon Hee Park; Zhengyan Kan; Wonshik Han; Woong-Yang Park
• Keywords: Tumour heterogeneity; Single-Cell Analysis

Abstract Single-cell transcriptome profiling of tumour tissue isolates allows the characterization heterogeneous cells along with neighbouring stromal and immune cells. Here we adopt this powerful approach to breast cancer analyse 515 from 11 patients. Inferred copy number variations single-cell RNA-seq data separate carcinoma non-cancer At a resolution, display common signatures within as well intratumoral heterogeneity regarding subtype crucial cancer-related pathways. Most are cells, three distinct clusters T lymphocytes, B lymphocytes macrophages. macrophages both immunosuppressive characteristics: regulatory or an exhausted phenotype M2 phenotype. These results illustrate that has wide range heterogeneity, which is shaped by in surrounding microenvironment.