High-resolution analysis of the human T-cell receptor repertoire

• DOI: 10.1038/ncomms9081
• Publication Date: 2015-09-01T10:04:14Z
• Authors: Eliana Ruggiero; Jan P. Nicolay; Raffaele Fronza; Anne Arens; Anna Paruzynski; Ali Nowrouzi; Gökçe Ürenden; Christina Lulay; Sven Schneider; Sergij Goerdt; Hanno Glimm; Peter H. Krammer; Manfred Schmidt; Christof von Kalle
• Keywords: Adult; Male; 0303 health sciences; Receptors, Antigen, T-Cell, alpha-beta; Receptors, Antigen, T-Cell; Genetic Variation; High-Throughput Nucleotide Sequencing; In Vitro Techniques; Middle Aged; Flow Cytometry; Polymerase Chain Reaction; Article; Lymphoma, T-Cell, Cutaneous; 3. Good health; Jurkat Cells; Mice; 03 medical and health sciences; Cytomegalovirus Infections; Genes, T-Cell Receptor beta; Animals; Humans; Female; Genes, T-Cell Receptor alpha; Aged

Abstract Unbiased dissection of T-cell receptor (TCR) repertoire diversity at the nucleotide level could provide important insights into human immunity. Here we show that TCR ligation-anchored-magnetically captured PCR (TCR-LA-MC PCR) identifies α- and β-chain without sequence-associated or quantitative restrictions in healthy diseased conditions. TCR-LA-MC convergent recombination events, classifies different stages cutaneous lymphoma vivo demonstrates reactivation after vitro cytomegalovirus stimulation. allows ultra-deep data access to both physiological mechanisms influencing clonality all clinical settings with restricted distorted repertoires.