Adipocyte Differentiation‐related Protein in Human Skeletal Muscle: Relationship to Insulin Sensitivity

• DOI: 10.1038/oby.2005.160
• Publication Date: 2008-04-21T21:55:02Z
• Authors: Susan A. Phillips; Charles C. Choe; Theodore P. Ciaraldi; Andrew S. Greenberg; Alice P. S. Kong; Sunita C. Baxi; Louis Christiansen; Sunder R. Mudaliar; Robert R. Henry
• Keywords: Adult; Male; 2. Zero hunger; 0301 basic medicine; Membrane Proteins; Middle Aged; Lipid Metabolism; Perilipin-2; 3. Good health; 03 medical and health sciences; Diabetes Mellitus, Type 2; Adipocytes; Humans; Female; Obesity; Insulin Resistance; Muscle, Skeletal; Aged

Abstract Objective : To determine whether adipocyte differentiation‐related protein (ADRP), a lipid droplet—associated that binds to and sequesters intracellular fatty acids, is 1) expressed in human skeletal muscle 2) differentially regulated obtained from obese non‐diabetic (OND) diabetic (OD) subjects. Research Methods Procedures Ten OND subjects 15 OD underwent weight loss or pharmacological intervention program improve insulin sensitivity. Anthropometric data, hemoglobin A 1C , fasting glucose, lipids, glucose disposal rate were determined at baseline completion of studies. Biopsies the vastus lateralis (SkM) state Protein expression was by Western blotting. Results ADRP highly SkM (4.4 ± 1.54 AU/10 μg, protein, n = 10) (5.02 1.33 12) undergoing had decreased triglyceride levels improved action. content increased with 5.14 2.15 μg 9.92 1.57 ( p < 0.025). treated either troglitazone metformin, together glyburide, for 3 4 months. Both treatments attained similar glycemic control. lower (2.85 1.07 6) displayed up‐regulation (to 9.27 2.76 Discussion predominant SkM, low up‐regulated circumstances tolerance. Up‐regulation may act sequester acids as triglycerides discrete droplets could protect detrimental effects on action