Extracellular vesicles from pluripotent stem cell-derived mesenchymal stem cells acquire a stromal modulatory proteomic pattern during differentiation

• DOI: 10.1038/s12276-018-0142-x
• Publication Date: 2018-09-10T03:37:20Z
• Authors: Alejandro La Greca; Claudia Solari; Veronica Furmento; Antonella Lombardi; Maria Celeste Biani; Cyntia Aban; Lucia Moro; Marcela García; Alejandra Sonia G...; Gustavo Emilio Se...; Santiago Gabriel ...; Carlos Luzzani
• Keywords: Regenerative Medicine; Proteome

Mesenchymal stem/stromal cells (MSCs) obtained from pluripotent stem (PSCs) constitute an interesting alternative to classical MSCs in regenerative medicine. Among their many mechanisms of action, MSC extracellular vesicles (EVs) are a potential suitable substitute for future cell-free-based therapeutic approaches. Unlike cells, EVs do not elicit acute immune rejection, and they can be produced large quantities stored until ready use. Although the has already been proven, thorough characterization is lacking. In this work, we used label-free liquid chromatography tandem mass spectrometry proteomic approach identify most abundant proteins that secreted derived PSCs (PD-MSCs) parental induced (iPSCs). Next, compared both datasets found while iPSC enclose modulate RNA microRNA stability protein sorting, PD-MSC rich organize matrix, regulate locomotion, influence cell-substrate adhesion. Moreover, respective iPSCs share greater proportion proteins, proteome appears more specific. Correlation principal component analysis consistently aggregate but segregate EVs. Altogether, these findings suggest during differentiation, with EVs, acquire specific set proteins; arguably, difference might confer properties.