Low intensity near-infrared light promotes bone regeneration via circadian clock protein cryptochrome 1

• DOI: 10.1038/s41368-022-00207-y
• Publication Date: 2022-11-14T08:08:49Z
• Authors: Jinfeng Peng; Jiajia Zhao; Qingming Tang; Jinyu Wang; Wencheng Song; Xiaofeng Lu; Xiaofei Huang; Guangjin Chen; Wenhao Zheng; Luoying Zhang; Yunyun Han; Chunze Yan; Qian Wan; Lili Chen
• Keywords: RUNX2; Bone remodeling

Abstract Bone regeneration remains a great clinical challenge. Low intensity near-infrared (NIR) light showed strong potential to promote tissue regeneration, offering promising strategy for bone defect regeneration. However, the effect and underlying mechanism of NIR on remain unclear. We demonstrated that in rat skull model was significantly accelerated with low-intensity stimulation. In vitro studies stimulation could osteoblast differentiation mesenchymal stem cells (BMSCs) MC3T3-E1 cells, which associated increased ubiquitination core circadian clock protein Cryptochrome 1 (CRY1) nucleus. found reduction CRY1 induced by activated morphogenetic (BMP) signaling pathways, promoting SMAD1/5/9 phosphorylation increasing expression levels Runx2 Osterix . treatment may act through sodium voltage-gated channel Scn4a , be responder accelerate Together, these findings suggest situ CRY1-dependent manner, providing novel, efficient non-invasive defects.