High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging

• DOI: 10.1038/s41377-018-0094-x
• Publication Date: 2018-11-21T10:23:14Z
• Authors: Sergey Turtaev; Ivo T. Leite; Tristan Altwegg-B...; Janelle M. P. Pakan; Nathalie L. Roche...; Tomáš Čižmár
• Keywords: Microscopy; Letter; Fibre optics and optical communications; /dk/atira/pure/subjectarea/asjc/2500/2504; /dk/atira/pure/subjectarea/asjc/3100/3107; 610; FOS: Physical sciences; 600; Physics - Medical Physics; name=Electronic; name=Atomic and Molecular Physics; ddc:530; Biophotonics; Optical and Magnetic Materials; Medical Physics (physics.med-ph); and Optics; Adaptive optics; Physics - Optics; Optics (physics.optics)

Progress in neuroscience constantly relies on the development of new techniques to investigate complex dynamics neuronal networks. An ongoing challenge is achieve minimally-invasive and high-resolution observations activity vivo inside deep brain areas. A perspective strategy utilise holographic control light propagation media, which allows converting a hair-thin multimode optical fibre into an ultra-narrow imaging tool. Compared current endoscopes based GRIN lenses or bundles, this concept offers footprint reduction exceeding order magnitude, together with significant enhancement resolution. We designed compact high-speed system for fluorescent at tip fibre, achieving micron-scale resolution across 50 um field view, yielding 7-kilopixel images rate 3.5 frames/s. Furthermore, we demonstrate cell bodies processes inhibitory neurons within layers visual cortex hippocampus anesthetised mice. This study forms basis several modern microscopy be delivered tissue living animal models while causing minimal impact its structural functional properties.