Age-associated antigen-presenting cell alterations promote dry-eye inducing Th1 cells

• DOI: 10.1038/s41385-018-0127-z
• Publication Date: 2019-01-29T12:04:31Z
• Authors: Fang Bian; Yangyan Xiao; Flavia L. Barbosa; Rodrigo G. de Souza; Humberto Hernandez; Zhiyuan Yu; Stephen C. Pflugf...; Cintia S. de Paiva
• Keywords: CXCR3; CD86; Adoptive Cell Transfer

Aging is a significant risk factor for dry eye. Here we used murine aging model to investigate the effects of on antigen presenting cells (APCs) and generation pathogenic T helper (Th)-1 cells. Our results showed that APCs from aged mice accumulate at conjunctiva, have higher levels co-activation marker CD86 lower aldehyde dehydrogenase activity. Using topical ovalbumin peptide as surrogate antigen, observed an increased number antigen-loaded in draining cervical lymph nodes group loss tight junction protein occludin conjunctiva. Aged greater Th1 than young antigen-presentation assays vitro. lacrimal glands, accumulation IFN-γ producing CD4+T cells, while Th-17 were present only nodes. There was also age-related increase CD4+CXCR3+IFN-γ+ nodes, glands CD4+CCR6+IL-17A+ mice. Adoptive transfer CD4+CXCR3+ into young, naive immunodeficient recipients caused goblet cell donor demonstrate age-associated changes are critical pathogenesis