Characterisation of peri-implantation endometrial Treg and identification of an altered phenotype in recurrent pregnancy loss

• DOI: 10.1038/s41385-021-00451-1
• Publication Date: 2021-09-22T10:03:10Z
• Authors: Ingrid Granne; Mengni Shen; Helena Rodriguez-...; Gurmeher Chadha; Elizabeth O'Donnell; Jan J. Brosens; Siobhan Quenby; Tim Child; Jennifer H. South...
• Keywords: Recurrent miscarriage; Decidua; Early Pregnancy Loss

Recurrent Pregnancy Loss (RPL) affects 2-4% of couples, and with increasing numbers pregnancy losses the risk miscarrying a euploid is increased, suggesting RPL pathology distinct from sporadic miscarriage that due largely to lethal embryonic aneuploidy. There are number conditions associated including unspecified "immune" pathologies; one strongest candidates for dysregulation remains T regulatory cells as depletion in very early stages mice leads loss. Human endometrial Treg conventional CD4T were isolated during peri-implantation period menstrual cycle normal women. We identified an transcriptomic signature validated enhanced phenotype compared peripheral blood Treg. Parous women had altered transcriptome nulliparity, indicating acquired immune memory within population, by comparison not altered. primary secondary nulliparous or parous controls respectively. Both subgroups displayed differentially expressed gene transcriptomes controls. found increased cell surface S1PR1 decreased TIGIT protein expression RPL, confirming presence endometrium.