Long noncoding RNA TUG1 facilitates osteogenic differentiation of periodontal ligament stem cells via interacting with Lin28A

• DOI: 10.1038/s41419-018-0484-2
• Publication Date: 2018-04-19T07:55:59Z
• Authors: Qin He; Shuangyan Yang; Xiuge Gu; Mengying Li; Chunling Wang; Fulan Wei
• Keywords: RUNX2

Abstract Periodontal ligament stem cells (PDLSCs) are mesenchymal derived from dental tissues with multidirectional differentiation potential and excellent self-renewing ability. Recently, long noncoding RNAs (lncRNAs) have been shown to play important roles in MSC osteogenic differentiation. In this study, we found that taurine upregulated gene 1 (TUG1), an evolutionarily conserved widely present lncRNA was significantly osteogenically induced PDLSCs compared their undifferentiated counterparts. Further investigation demonstrated the expression of TUG1 positively correlated following induction, as evidenced by increase cellular alkaline phosphatase (ALP) level, formation calcium nodules, upregulation several osteogenic-related markers such ALP, osteocalcin (OCN), runt-related transcription factor 2 (Runx2). Conversely, knockdown inhibit for Using bioinformatics analysis, identified lin-28 homolog A (Lin28A) a target during PDLSCs. Lin28A be downregulated TUG1-repressed contained multiple binding sites TUG1. Moreover, suppression able decreased genes. Taken together, these results could help researchers better understand mechanism governs PDLSCs, also serve stepping stone development novel therapeutic strategies can used regenerate tissues.